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In the present study, the effects of transplanted allografts on immune cells were assessed by labeling with in vivo, perivascular macrophage-specific MR contrast agent and ex vivo T2*-weighted heart imaging by MRM. The signal intensity of MRI signal at rejecting and non-rejecting sites was significantly different, clearly demonstrating an in vivo immune cell accumulation in the rejecting sites. In addition, the macrophage-specific MR contrast agent allowed us to detect focal areas of macrophage infiltration pre-emptively and immediately in the rejection process by T2*-weighted imaging, prior to the development of regional histological abnormalities.

Conclusions: With this study, in vivo detection of immune cell infiltration into transplanted allografts was attempted, and the results showed that MRM is capable of identifying macrophage infiltration from within at an early stage of allograft rejection. When using this modality, the overt allograft rejection detected may not be clinically significant. However, it may be possible to detect subclinical immune cell infiltration and, with detailed analysis, predict such events to occur at a point in time when they may be of greatest prognostic value.

Magnetic resonance imaging, which is capable of noninvasive, non-ionizing and non-radiation imaging, has recently been applied to a variety of medical problems, including clinical image diagnosis and forensic medicine.

Although the main imaging modalities for depicting tissue motion involve the change in the absorption of electromagnetic waves, such as X-rays and ultrasound, the dynamic contrast-enhanced MRI technique uses spin-echo sequences to measure signals resulting from the contributions of paramagnetic and diamagnetic particles to magnetic field loss through the time-dependent behavior of the particles, allowing the depiction of the distribution of blood vessels and tumors. This technique is widely used for the assessment of biological function and measurement of the blood flow in arterial vessels of the brain, heart, kidney, and other tissues.


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